Proliferative activity and expression of cyclin-dependent kinase inhibitor p21WAF1 and p53 protein in endothelial cells of human aorta during replicative aging in vitro

Yu G Antropova; A L Bryantsev; N I Kalinina; O P Il'inskaya; E M Tararak

doi:10.1023/a:1020677209755

Proliferative activity and expression of cyclin-dependent kinase inhibitor p21WAF1 and p53 protein in endothelial cells of human aorta during replicative aging in vitro

Bull Exp Biol Med. 2002 Jul;134(1):81-3. doi: 10.1023/a:1020677209755.

Authors

Yu G Antropova¹, A L Bryantsev, N I Kalinina, O P Il'inskaya, E M Tararak

Affiliation

¹ Laboratory of Molecular and Cellular Cardiology, Institute of Experimental Cardiology, Russian Research-and-Production Center for Cardiology, Russian Ministry of Health, Moscow.

PMID: 12459877
DOI: 10.1023/a:1020677209755

Abstract

Experiments with bromodeoxyuridine showed that the count of nonproliferating cells in a monolayer culture of aortic endothelial cells from adult humans rapidly increased during long-term subculturing. Cytochemical assay showed that these cells contain neutral b-galactosidase, the marker of aging cells. Immunocytochemical assay demonstrated that most cells express p53 protein and inhibitor of the cell cycle p21WAF1.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Adult
Aging*
Aorta / cytology
Aorta / metabolism*
Bromodeoxyuridine / pharmacology
Cell Division
Cell Nucleus / metabolism
Cyclin-Dependent Kinase Inhibitor p21
Cyclins / biosynthesis*
Endothelium, Vascular / cytology
Endothelium, Vascular / metabolism*
Humans
Immunohistochemistry
In Vitro Techniques
Reactive Oxygen Species
Time Factors
Tumor Suppressor Protein p53 / biosynthesis*
beta-Galactosidase / metabolism

Substances

CDKN1A protein, human
Cyclin-Dependent Kinase Inhibitor p21
Cyclins
Reactive Oxygen Species
Tumor Suppressor Protein p53
beta-Galactosidase
Bromodeoxyuridine