Research has shown that adolescents from low-income communities receive less educational resources and support and may possess insufficient knowledge of nutrition and sport supplements to make health conscious decisions. The purpose of this investigation was to determine the effectiveness of a nutrition supplement educational intervention in improving the nutrition supplement knowledge of adolescents from a low-income community. Thirty-nine high school students from a low-income community were separated into experimental (E) (n = 22) and control (C) (n = 17) groups. E received 5 nutrition lessons on various nutrition and sport supplement topics. C did not receive any nutrition education. E consisted of 5 freshmen, 5 sophomores, 6 juniors, and 6 seniors. The ethnic backgrounds of E were: 15 African-Americans, 3 Hispanic-Americans, and 4 "others." There were 14 females and 8 males in E. C consisted of 9 freshmen, 1 sophomore, 4 juniors, and 3 seniors. The ethnic backgrounds of C were: 14 African-Americans, 1 Hispanic-American, and 2 "others." There were 10 females and 7 males in C. Both groups were administered a validated nutrition and sport supplement questionnaire at baseline and post-intervention that consisted of 28 questions. Mean baseline knowledge scores for E and C were 5.73 +/- 3.34 and 8.18 +/- 3.82, respectively (p = 0.040). Post-intervention scores were: 14.45 +/- 4.81 and 7.82 +/- 4.79 for E and C, respectively (p = 0.0001). These results indicate that a short-term nutrition education program can significantly improve nutrition and sport supplement knowledge in high school students of low socioeconomic status.