Abstract
Here we report the construction of three different vectors for the identification of bacterial genes induced in vitro and/or in vivo. These plasmids contain kanamycin, gentamicin, or tetracycline resistance genes as selectable markers. A promoterless cat and an improved GFP (mut3-gfp) can be used to follow the induction of gene expression by measuring chloramphenicol resistance and fluorescence, respectively.
MeSH terms
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Chloramphenicol O-Acetyltransferase / genetics
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Chromosomes, Bacterial / genetics
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Cloning, Molecular
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Conjugation, Genetic / genetics
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DNA Helicases / genetics
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DNA-Binding Proteins*
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Drug Resistance, Bacterial / genetics
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Extrachromosomal Inheritance
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Fluorometry
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Gene Expression Regulation, Bacterial
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Genes, Bacterial
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Genes, Reporter
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Genetic Vectors / genetics*
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Genomics
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Gram-Negative Bacteria / genetics*
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Green Fluorescent Proteins
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Luminescent Proteins / genetics
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Nitrogen Fixation / genetics
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Promoter Regions, Genetic / genetics*
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Rhizobium / genetics
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Trans-Activators / genetics
Substances
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DNA-Binding Proteins
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Luminescent Proteins
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Trans-Activators
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replication initiator protein
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Green Fluorescent Proteins
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Chloramphenicol O-Acetyltransferase
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DNA Helicases