This study examined the effects of four high-intensity interval-training (HIT) sessions performed over 2 weeks on peak volume of oxygen uptake (VO2peak), the first and second ventilatory thresholds (VT1, VT2) and peak power output (PPO) in highly trained cyclists. Fourteen highly trained male cyclists (VO2peak = 67.5 +/- 3.7 ml.kg-1.min-1) performed a ramped cycle test to determine VO2peak, VT1, VT2, and PPO. Subjects were divided equally into a HIT group and a control group. The HIT group performed four HIT sessions (20 x 60 s at PPO, 120 s recovery); the VO2peak test was repeated < 1 wk after the HIT program. Control subjects maintained their regular training program and were reassessed under the same timeline. There was no change in VO2peak for either group; however, the HIT group showed a significantly greater increase in VT1 (+22% vs. -3%), VT2 (+15% vs. -1%), and PPO (+4.3 vs. -.4%) compared to controls (all P < .05). This study has demonstrated that HIT can improve VT1, VT2, and PPO, following only four HIT sessions in already highly trained cyclists.