Abstract
Seven Klebsiella pneumoniae and four Klebsiella oxytoca clinical isolates with different levels of resistance to ciprofloxacin were studied. Mutations in the topoisomerase genes were found in almost all strains, but the contribution of a multidrug efflux system homologous to AcrAB in Escherichia coli was also observed. Overexpression of this efflux system was demonstrated by immunoblotting with antibodies against E. coli AcrA.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Anti-Infective Agents / pharmacology*
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Ciprofloxacin / metabolism
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Ciprofloxacin / pharmacology
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DNA Topoisomerases, Type I / genetics
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Drug Resistance, Multiple, Bacterial / genetics*
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Klebsiella oxytoca / drug effects
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Klebsiella oxytoca / genetics*
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Klebsiella oxytoca / metabolism
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Klebsiella pneumoniae / drug effects
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Klebsiella pneumoniae / genetics*
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Klebsiella pneumoniae / metabolism
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Microbial Sensitivity Tests
Substances
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Anti-Infective Agents
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Ciprofloxacin
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DNA Topoisomerases, Type I