Formation of phosphine and methane in anaerobic incubation systems was investigated under stirred and unstirred conditions. The PH3 and CH4 levels in the headspace, as well as the matrix-bound PH3 content in the stirred soil, significantly increased upon the addition of phosphonoacetic acid (P(O)(OH)2CH2COOH). Both the levels of matrix-bound PH3 and CH4 are positively correlated to the buffered dithionite fraction of reactive phosphorus in the soil samples, while a negative correlation was observed between matrix-bound PH3/CH4 levels and the reactive phosphorus fraction.