Tyrosine 92 of aristolochene synthase directs cyclisation of farnesyl pyrophosphate

Melanie J Calvert; Susan E Taylor; Rudolf K Allemann

doi:10.1039/b206517g

Tyrosine 92 of aristolochene synthase directs cyclisation of farnesyl pyrophosphate

Chem Commun (Camb). 2002 Oct 21:(20):2384-5. doi: 10.1039/b206517g.

Authors

Melanie J Calvert¹, Susan E Taylor, Rudolf K Allemann

Affiliation

¹ School of Chemical Sciences, University of Birmingham, Edgbaston, Birmingham, UK B15 2TT.

PMID: 12430452
DOI: 10.1039/b206517g

Abstract

A mutant of Aristolochene Synthase (AS), in which Tyr 92 was replaced by Val, produced the alicyclic beta-(E)-farnesene as the major product, indicating that cyclisation of FPP is controlled by Tyr 92 in AS.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Amino Acid Substitution
Cyclization / drug effects
Gas Chromatography-Mass Spectrometry
Isomerases / chemistry
Isomerases / genetics
Isomerases / metabolism*
Mutagenesis, Site-Directed
Polyisoprenyl Phosphates / metabolism*
Sesquiterpenes / metabolism
Tyrosine* / pharmacology

Substances

Polyisoprenyl Phosphates
Sesquiterpenes
beta-farnesene
Tyrosine
farnesyl pyrophosphate
Isomerases
aristolochene synthase