Synthesis of long Poly(dA).Poly(dT) DNA without structural defects using enzymatic reaction; tailored ligated Poly(dA).Poly(dT)

Shin-ichi Tanaka; Shinsuke Fujiwara; Hiroyuki Tanaka; Masateru Taniguchi; Hitoshi Tabata; Kiichi Fukui; Tomoji Kawai

doi:10.1039/b205821a

Synthesis of long Poly(dA).Poly(dT) DNA without structural defects using enzymatic reaction; tailored ligated Poly(dA).Poly(dT)

Chem Commun (Camb). 2002 Oct 21:(20):2330-1. doi: 10.1039/b205821a.

Authors

Shin-ichi Tanaka¹, Shinsuke Fujiwara, Hiroyuki Tanaka, Masateru Taniguchi, Hitoshi Tabata, Kiichi Fukui, Tomoji Kawai

Affiliation

¹ Institute of Scientific and Industrial Research, Osaka University, 8-1 Mihogaoka, Ibaraki, Osaka 567-0047, Japan.

PMID: 12430426
DOI: 10.1039/b205821a

Abstract

Poly(dA).Poly(dT) molecules up to 1000 base pairs (bp) have been synthesized using enzymatic reaction, and characterization by STM observation shows that the DNA has no defects in the duplex structure.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

DNA-Directed DNA Polymerase / chemistry
Electrophoresis, Agar Gel
Microscopy, Electron, Scanning Transmission
Nucleic Acid Conformation
Poly dA-dT / chemical synthesis*
Poly dA-dT / chemistry
Polynucleotide 5'-Hydroxyl-Kinase / chemistry
RNA Ligase (ATP) / chemistry
Single-Strand Specific DNA and RNA Endonucleases / chemistry

Substances

Poly dA-dT
Polynucleotide 5'-Hydroxyl-Kinase
DNA-Directed DNA Polymerase
Single-Strand Specific DNA and RNA Endonucleases
RNA Ligase (ATP)