This study was undertaken in rats to study the influence of the suprahyoid muscles on mandibular growth, morphology, and orientation. The aim was to investigate the effect of bilateral suprahyoid muscle myectomy on the skeletal growth and orientation of the mandible and on its orientation in rats. Forty-eight 4-week-old rats were divided into 2 experimental and 2 control groups as follows: A, 12 animals in which bilateral excision of the anterior digastric muscle was performed; B, 12 animals in which bilateral excision of the anterior digastric, transverse mandibular, and mylohyoid muscles was performed; C, 12 animals that were sham-operated bilaterally without any muscular excision; D, 12 control animals not subjected to any operation. The experimental period was 30 days. Lateral and dorsoventral radiographs were taken on days 1 and 30, after muscular excision. Cephalometric analysis was performed for each animal according to a method and procedure established in our laboratory and described earlier. The findings support the occurrence of decreased mandibular growth in the group in which bilateral digastric, transverse mandibular, and mylohyoid excision was performed as compared with controls. In addition, the mandible in the bilateral digastric excision group exhibited a more upward orientation. It was thus proven that the absence of the suprahyoid musculature does affect both skeletal growth and orientation of the mandible.