Expression and folding of an antibody fragment selected in vivo for high expression levels in Escherichia coli cytoplasm

Jean-Charles Laden; Pascal Philibert; François Torreilles; Martine Pugnière; Pierre Martineau

doi:10.1016/s0923-2508(02)01347-5

Expression and folding of an antibody fragment selected in vivo for high expression levels in Escherichia coli cytoplasm

Res Microbiol. 2002 Sep;153(7):469-74. doi: 10.1016/s0923-2508(02)01347-5.

Authors

Jean-Charles Laden¹, Pascal Philibert, François Torreilles, Martine Pugnière, Pierre Martineau

Affiliation

¹ CNRS UMR5094, Faculté de Pharmacie, Montpellier, France.

PMID: 12405355
DOI: 10.1016/s0923-2508(02)01347-5

Abstract

In this review, we summarize some of our results on folding and directed evolution of an antibody fragment in Escherichia coli cytoplasm. We will also discuss some attempts to construct other antibodies active in this cellular compartment.

Publication types

Research Support, Non-U.S. Gov't
Review

MeSH terms

Cytoplasm / metabolism*
Directed Molecular Evolution*
Escherichia coli / genetics*
Escherichia coli / metabolism
Immunoglobulin Fragments / genetics
Immunoglobulin Fragments / metabolism*
Protein Folding*

Substances

Immunoglobulin Fragments
immunoglobulin Fv