Combined use of the fully automated Bactec MGIT 960 system and a PCR-restriction fragment length polymorphism analysis for routine detection and identification of mycobacteria from clinical samples

Fanourios Kontos; Efthymia Petinaki; Zoe Gitti; Christos Costopoulos; Spyridoula Anagnostou; Ioannis Tselentis; Antonios N Maniatis

doi:10.1016/s0167-7012(02)00137-9

Combined use of the fully automated Bactec MGIT 960 system and a PCR-restriction fragment length polymorphism analysis for routine detection and identification of mycobacteria from clinical samples

J Microbiol Methods. 2003 Jan;52(1):137-40. doi: 10.1016/s0167-7012(02)00137-9.

Authors

Fanourios Kontos¹, Efthymia Petinaki, Zoe Gitti, Christos Costopoulos, Spyridoula Anagnostou, Ioannis Tselentis, Antonios N Maniatis

Affiliation

¹ Department of Microbiology, Medical School, University Hospital of Larissa, Papakyriazi 22, Larissa, Greece.

PMID: 12401236
DOI: 10.1016/s0167-7012(02)00137-9

Abstract

The combined use of Bactec MGIT 960 system and a PCR-Restriction Fragment Length Polymorphism (PCR-RFLP) analysis was assessed for the rapid detection and identification of mycobacteria from clinical samples. The diagnostic sensitivity and the time of detection of MGIT 960 system were significantly higher than of Löwenstein-Jensen medium. PCR-RFLP identification analysis results were in concordance with those obtained by the conventional biochemical tests.

MeSH terms

Bacteriological Techniques / instrumentation*
Humans
Mycobacterium Infections / diagnosis
Mycobacterium Infections / microbiology
Mycobacterium tuberculosis / growth & development
Mycobacterium tuberculosis / isolation & purification*
Polymerase Chain Reaction / methods*
Polymorphism, Restriction Fragment Length
Reagent Kits, Diagnostic
Sensitivity and Specificity

Substances

Reagent Kits, Diagnostic