A new digalactosyl diacylglycerol (1) has been isolated from the cultured marine dinoflagellate Heterocapsa circularisquama together with known monogalactosyl diacylglycerols 2 and 3, and their structures were elucidated by spectroscopic methods. The digalactosyl diacylglycerol 1 showed cytolytic activity toward heart cells of oysters.