Effective artificial proteases synthesized by covering silica gel with aldehyde and various other organic groups

Bioorg Med Chem Lett. 2002 Nov 18;12(22):3247-50. doi: 10.1016/s0960-894x(02)00724-2.

Abstract

Organic artificial proteases with broad substrate specificity were synthesized by covering the surface of silica gel with aldehyde and the functional groups present in amino acids. The artificial proteases hydrolyzed ovalbumin, albumin, hemoglobin, gamma-globulin with half-lives as short as 50 min at 25 degrees C or 7 min at 50 degrees C.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Aldehydes / chemistry*
  • Animals
  • Catalysis
  • Endopeptidases / chemical synthesis*
  • Endopeptidases / chemistry
  • Humans
  • Hydrolysis
  • Kinetics
  • Molecular Mimicry
  • Organic Chemicals / chemistry
  • Proteins / chemistry
  • Proteins / metabolism
  • Silica Gel
  • Silicon Dioxide
  • Substrate Specificity

Substances

  • Aldehydes
  • Organic Chemicals
  • Proteins
  • Silica Gel
  • Silicon Dioxide
  • Endopeptidases