[Expression of polyprotein of infectious bursal disease virus in Bombyx mori]

Sheng Wu Gong Cheng Xue Bao. 2002 Jul;18(4):472-6.
[Article in Chinese]

Abstract

Segment A of the genome of infectious bursal disease virus(IBDV) encodes structure protein VP2 and VP3 and protease VP4. In this study a polyprotein gene of IBDV was inserted into a Bombyx mori baculovirus transfer vector pAcHLT--C and contransfected into BmN cells with linear genome DNA of virus Bm-BacPAK6. Dot hybridization suggested that the segment A of the virus genome was inserted in the genome of Bm-BacPAK6. The silkworm of fifth instars were infected by the recombinant virus and the immunogenicity of the infected larvae's blood were examined with ELISA, SDS-PAGE and Western blotting. It appears that the recombinant polyprotein has the property of immunoreactivity and the expression in larvae reached the pick 5-day post infection.

Publication types

  • English Abstract
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Blotting, Western
  • Bombyx / genetics*
  • Bombyx / virology
  • Electrophoresis, Polyacrylamide Gel
  • Enzyme-Linked Immunosorbent Assay
  • Gene Expression
  • Genetic Vectors / genetics
  • Infectious bursal disease virus / genetics*
  • Polyproteins / genetics*
  • Polyproteins / metabolism
  • Viral Proteins / genetics
  • Viral Proteins / metabolism

Substances

  • Polyproteins
  • Viral Proteins