Abstract
Using PCR, we screened 105 isolates of poultry-associated Campylobacter jejuni for the presence of class 1 integrons. Of those isolates, 21% (22 of 105) possessed the integrase gene, but only 5 isolates produced an amplicon in a 5'-3' conserved sequence PCR directed toward amplification of the resistance cassettes. DNA sequencing demonstrated that all five isolates possessed the aminoglycoside resistance gene, aacA4.
MeSH terms
-
Animals
-
Anti-Bacterial Agents / pharmacology*
-
Campylobacter Infections / microbiology*
-
Campylobacter Infections / veterinary*
-
Campylobacter jejuni / drug effects*
-
Chickens / microbiology*
-
DNA Primers
-
DNA, Bacterial / analysis
-
DNA, Bacterial / genetics
-
Drug Resistance, Bacterial
-
Enzyme-Linked Immunosorbent Assay
-
Gentamicins / pharmacology*
-
Integrons / genetics*
-
Intestines / microbiology
-
Molecular Sequence Data
-
Poultry Diseases / microbiology*
-
Reverse Transcriptase Polymerase Chain Reaction
-
Tobramycin / pharmacology*
Substances
-
Anti-Bacterial Agents
-
DNA Primers
-
DNA, Bacterial
-
Gentamicins
-
Tobramycin
Associated data
-
GENBANK/AF439785
-
GENBANK/AF439786