Class 1 integron-associated tobramycin-gentamicin resistance in Campylobacter jejuni isolated from the broiler chicken house environment

Antimicrob Agents Chemother. 2002 Nov;46(11):3660-4. doi: 10.1128/AAC.46.11.3660-3664.2002.

Abstract

Using PCR, we screened 105 isolates of poultry-associated Campylobacter jejuni for the presence of class 1 integrons. Of those isolates, 21% (22 of 105) possessed the integrase gene, but only 5 isolates produced an amplicon in a 5'-3' conserved sequence PCR directed toward amplification of the resistance cassettes. DNA sequencing demonstrated that all five isolates possessed the aminoglycoside resistance gene, aacA4.

MeSH terms

  • Animals
  • Anti-Bacterial Agents / pharmacology*
  • Campylobacter Infections / microbiology*
  • Campylobacter Infections / veterinary*
  • Campylobacter jejuni / drug effects*
  • Chickens / microbiology*
  • DNA Primers
  • DNA, Bacterial / analysis
  • DNA, Bacterial / genetics
  • Drug Resistance, Bacterial
  • Enzyme-Linked Immunosorbent Assay
  • Gentamicins / pharmacology*
  • Integrons / genetics*
  • Intestines / microbiology
  • Molecular Sequence Data
  • Poultry Diseases / microbiology*
  • Reverse Transcriptase Polymerase Chain Reaction
  • Tobramycin / pharmacology*

Substances

  • Anti-Bacterial Agents
  • DNA Primers
  • DNA, Bacterial
  • Gentamicins
  • Tobramycin

Associated data

  • GENBANK/AF439785
  • GENBANK/AF439786