No-tillage (NT) is becoming increasingly attractive to farmers because it clearly reduces soil erosion and production costs relative to conventional tillage (CT). However, the impacts of no-tillage on the quantity and quality of tile drainage water are less well known. Accordingly, two adjacent field scale on-farm CT and NT sites were established to compare the impacts of the two tillage systems on tile drainage and NO3-N loss in tile drainage water. The effect of the two tillage systems on soil structure, hydraulic conductivity, and earthworm population were also investigated. The total NO3-N loss in tile drainage water over the 5-yr period (1995-1999) was 82.3 kg N ha(-1) for the long-term NT site and 63.7 kg N ha(-1) for the long-term CT site. The long-term NT site had 48% more tile drainage (6,975 kL ha(-1)) than the long-term CT site (4,716 kL ha(-1)). The average flow weighted mean (FWM) NO3-N concentration in tile drainage water over the 5-yr period was 11.8 mg N L(-1) for the NT site and 13.5 mg N L(-1) for the CT site. For both tillage systems, approximately 80% of tile drainage and NO3-N loss in tile drainage water occurred during the November to April non-growing season. Long-term NT improved wet aggregate stability, increased near-surface hydraulic conductivity and increased both the number and mass of earthworms relative to long-term CT. The greater tile drainage and NO3-N loss under NT were attributed to an increase in continuous soil macropores, as implied by greater hydraulic conductivity and greater numbers of earthworms.