Determination of vegetal proteins in milk powder by sodium dodecyl sulfate-capillary gel electrophoresis: interlaboratory study

J AOAC Int. 2002 Sep-Oct;85(5):1090-5.

Abstract

An interlaboratory study, with the participation of 8 laboratories, was conducted to evaluate a sodium dodecyl sulfate-capillary gel electrophoresis method for determination of adulteration of milk powder with soy and pea proteins. Calibration standards (0-8%, w/w, soy and pea protein in total protein) and adulterated skim milk powders (0-5%, w/w, soy and pea proteins in total protein) were produced. Vegetal proteins were determined after removal of milk proteins by pretreatment of the samples with tetraborate-EDTA buffer, pH 8.3. Repeatability standard deviations ranged from 9 to 15% and reproducibility standard deviations ranged from 25 to 30% in the samples containing 5% vegetal protein in total protein.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Calibration
  • Dairy Products / analysis*
  • Electrophoresis, Capillary
  • Electrophoresis, Polyacrylamide Gel
  • Enzyme-Linked Immunosorbent Assay
  • Food Preservation
  • Indicators and Reagents
  • Plant Proteins / analysis*
  • Reference Standards
  • Reproducibility of Results

Substances

  • Indicators and Reagents
  • Plant Proteins