LNA-enhanced detection of single nucleotide polymorphisms in the apolipoprotein E

Nucleic Acids Res. 2002 Oct 1;30(19):e100. doi: 10.1093/nar/gnf099.

Abstract

Genotyping of single nucleotide polymorphisms (SNPs) in large populations presents a great challenge, especially if the SNPs are embedded in GC-rich regions, such as the codon 112 SNP in the human apolipoprotein E (apoE). In the present study, we have used immobilized locked nucleic acid (LNA) capture probes combined with LNA-enhancer oligonucleotides to obtain efficient and specific interrogation of SNPs in the apoE codons 112 and 158, respectively. The results demonstrate the usefulness of LNA oligonucleotide capture probes combined with LNA enhancers in mismatch discrimination. The assay was applied to a panel of patient samples with simultaneous genotyping of the patients by DNA sequencing. The apoE genotyping assays for the codons 112 and 158 SNPs resulted in unambiguous results for all patient samples, concurring with those obtained by DNA sequencing.

MeSH terms

  • Apolipoproteins E / genetics*
  • Base Sequence
  • DNA, Complementary / genetics
  • Genotype
  • Humans
  • Molecular Sequence Data
  • Nucleic Acid Denaturation
  • Nucleic Acid Hybridization / methods
  • Oligonucleotide Probes / chemistry
  • Oligonucleotide Probes / genetics*
  • Photochemistry
  • Polymorphism, Single Nucleotide / genetics*
  • Sensitivity and Specificity

Substances

  • Apolipoproteins E
  • DNA, Complementary
  • Oligonucleotide Probes