DNA differential diagnosis of human taeniid cestodes by base excision sequence scanning thymine-base reader analysis with mitochondrial genes

J Clin Microbiol. 2002 Oct;40(10):3818-21. doi: 10.1128/JCM.40.10.3818-3821.2002.

Abstract

For DNA differential diagnosis of human Taenia cestodes, a base excision sequence scanning thymine-base method using the cytochrome c oxidase subunit I and cytochrome b genes as targets was used. The characteristic thymine-base peak profiles provide four distinct types, unique for T. saginata, T. asiatica, and two genotypes of T. solium. This approach provides a useful tool for the identification and diagnosis of human taeniid cestodes without DNA sequencing if nucleotide sequence databases are available.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Cytochrome b Group / genetics
  • DNA, Helminth / analysis*
  • DNA, Mitochondrial / analysis*
  • Electron Transport Complex IV / genetics
  • Humans
  • Taenia saginata / enzymology
  • Taenia saginata / genetics
  • Taenia saginata / isolation & purification
  • Taenia solium / enzymology
  • Taenia solium / genetics
  • Taenia solium / isolation & purification
  • Taeniasis / diagnosis*
  • Taeniasis / parasitology

Substances

  • Cytochrome b Group
  • DNA, Helminth
  • DNA, Mitochondrial
  • Electron Transport Complex IV