Incorporation of a novel nucleobase allows stable oligonucleotide-directed triple helix formation at the target sequence containing a purine.pyrimidine interruption

Chem Commun (Camb). 2001 Sep 21:(18):1814-5. doi: 10.1039/b103743a.

Abstract

Thermal denaturation experiments have established that an oligonucleotide incorporating the artificial nucleobase S, does form a stable triplex with a double stranded DNA which exhibits a pyrimidine interruption within the oligopurine sequence.

MeSH terms

  • Base Sequence
  • DNA / chemistry*
  • DNA / metabolism*
  • Nucleic Acid Conformation*
  • Nucleic Acid Denaturation
  • Oligonucleotides / chemistry*
  • Oligonucleotides / metabolism*
  • Purines / metabolism
  • Pyrimidines / metabolism

Substances

  • Oligonucleotides
  • Purines
  • Pyrimidines
  • triplex DNA
  • DNA