Raphanus raphanistrum L has evolved widespread resistance to sulfonylureas in the Western Australia (WA) wheat belt. With the introduction of imidazolinone-tolerant (IT) wheat (Tritcum aestivum L) and IT canola (Brassica napus L) in the WA wheat belt, it is important to understand the status of cross-resistance in this weed to sulfonylurea and imidazolinone (Imi) herbicides. A study was conducted to examine cross-resistance between chlorsulfuron and Imi herbicides (a mixture of imazapic and imazapyr) in 46 R raphanistrum populations collected from across the WA wheat belt. Plants were treated with herbicides and assessed for phytotoxicity under glasshouse conditions. Of the 46 R raphanistrum populations, 32 were resistant to chlorsulfuron and four were resistant to imazapic + imazapyr. Of the 70% chlorsulfuron-resistant populations, 13% showed cross-resistance to imazapic + imazapyr. However, the cross-resistant populations treated with imazapic + imazapyr showed a lower resistance level than the chlorsulfuron-treated populations. These results suggest that weed populations with such cross-resistance will not be controlled effectively by Imi herbicides. Although the resistance levels of the cross-resistant populations to Imi herbicides were low, the cross-resistance levels of R raphanistrum should be determined before growing IT crops, particularly IT canola.