Abstract
Buccal mucosa carcinoma-derived cell line, HO-1-N-1, epithelial-like cells, was obtained in order to investigate the characteristics of oral cancer cells and examine the [Ca2+]i responses to stimulants, such as bradykinin (BK), histamine (HIST), thapsigargin (TG), epidermal growth factor (EGF) and transforming growth factor alpha (TGF alpha ). Intracellular Ca2+ influx was observed by all stimulants that enhanced the [Ca2+]i response. However, intracellular Ca2+ release was not observed in response to growth factors. The [Ca2+]i response of BK (100 nM) was inhibited by 10 micro M of the BKB2 antagonist, D-Arg-[Hyp3, Thi5,8, D-Phe7]-BK, and HIST (1 mM) was completely inhibited by 100 nM of the H1 antagonist, (+)-chlorpheniramine, in the presence and absence of extracellular Ca2+ (1.5 mM).
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Bradykinin / antagonists & inhibitors
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Bradykinin / pharmacology
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Calcium / metabolism*
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Calcium Signaling / drug effects*
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Carcinoma / metabolism*
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Carcinoma / pathology
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Epidermal Growth Factor / pharmacology
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Epithelial Cells / drug effects
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Epithelial Cells / metabolism*
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Histamine / pharmacology
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Histamine H1 Antagonists / pharmacology
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Humans
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Mouth Mucosa / pathology
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Mouth Neoplasms / metabolism*
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Mouth Neoplasms / pathology
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Receptors, Cell Surface / metabolism
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Stimulation, Chemical
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Thapsigargin / pharmacology
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Transforming Growth Factor alpha / pharmacology
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Tumor Cells, Cultured / drug effects
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Tumor Cells, Cultured / metabolism*
Substances
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Histamine H1 Antagonists
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Receptors, Cell Surface
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Transforming Growth Factor alpha
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Epidermal Growth Factor
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Thapsigargin
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Histamine
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Bradykinin
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Calcium