The equipment of using Azolla for O2-supplementation and food-production in future space station was developed and tested. Dog was used as the O2-consuming animal. The design of this device considered both the requirement of Azolla growth, such as illumination, temperature, humidity, nutrition and biomass harvesting, and also the food supplement, excretion draining and temperature controlling for the dog under the condition of an airtight chamber for a relatively long duration. This device was preliminarily tested for O2-release by Azolla, and data about O2-supplement by Azolla were obtained.