Optical interference artifacts in contact atomic force microscopy images

Ultramicroscopy. 2002 Aug;92(3-4):243-50. doi: 10.1016/s0304-3991(02)00140-7.

Abstract

Atomic force microscopy images are usually affected by different kinds of artifacts due to either the microscope design and operation mode or external environmental factors. Optical interferences between the laser light reflected off the top of the cantilever and the light scattered by the surface in the same direction is one of the most frequent sources of height artifact in contact (and occasionally non-contact) images. They are present when imaging highly reflective surfaces, or even when imaging non-reflective materials deposited onto reflective ones. In this study interference patterns have been obtained with a highly polished stainless steel planchet. The influence of these artifacts in surface roughness measurements is discussed, and a semi-quantitative method based on the fast Fourier transform technique is proposed to remove the artifacts from the images. This method improves the results obtained by applying the usual flattening routines.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Artifacts*
  • Fourier Analysis
  • Lenses
  • Microscopy, Atomic Force / instrumentation*
  • Microscopy, Atomic Force / methods*
  • Stainless Steel
  • Surface Properties
  • Yeasts / ultrastructure

Substances

  • Stainless Steel