Effect of NADH on the redox state of human hemoglobin

Clin Chim Acta. 2002 Oct;324(1-2):129-34. doi: 10.1016/s0009-8981(02)00242-5.

Abstract

In this work, we report that NADH can increase the autoxidation rate of hemoglobin (HbA) in a pH-dependent fashion. During this process, this cofactor is itself oxidized. The presence of superoxide dismutase (SOD) and/or catalase (CAT) can inhibit this result. At lower pH rates, the effect of NADH on the hemoglobin autoxidation rate is more enhanced; in addition, the rate of NADH oxidation is increased. Our data indicates that the reduced pyridine nucleotide may influence the redox state of human hemoglobin by a mechanism, which probably involves free radical species.

MeSH terms

  • Antioxidants / metabolism
  • Catalase / metabolism
  • Free Radicals / metabolism
  • Hemoglobins / metabolism*
  • Humans
  • Hydrogen-Ion Concentration
  • Kinetics
  • NAD / metabolism*
  • Oxidants / metabolism
  • Oxidation-Reduction
  • Superoxide Dismutase / metabolism

Substances

  • Antioxidants
  • Free Radicals
  • Hemoglobins
  • Oxidants
  • NAD
  • Catalase
  • Superoxide Dismutase