Tag-reporter and resin capture--release strategy in oligosaccharide synthesis

Yukishige Ito; Shino Manabe

doi:10.1002/1521-3765(20020715)8:14<3076::AID-CHEM3076>3.0.CO;2-N

Tag-reporter and resin capture--release strategy in oligosaccharide synthesis

Chemistry. 2002 Jul 15;8(14):3076-84. doi: 10.1002/1521-3765(20020715)8:14<3076::AID-CHEM3076>3.0.CO;2-N.

Authors

Yukishige Ito¹, Shino Manabe

Affiliation

¹ RIKEN, The Institute of Physical and Chemical Research, Wako-shi, Saitama, Japan. yukito@postman.riken.go.jp

PMID: 12203337
DOI: 10.1002/1521-3765(20020715)8:14<3076::AID-CHEM3076>3.0.CO;2-N

Abstract

Low molecular weight poly(ethylene glycol) (LWPEG) has been found to be useful as a "tag" in oligosaccharide synthesis due to its high polarity. Real-time reaction monitoring was achieved by use of MALDI-TOF MS in the glycosylation reactions and color tests in the deprotection of the chloroacetyl group. Further, a cysteine-supported insoluble resin enables the purification of the chloroacetyl-bound compounds on soluble PEG.

Publication types

Research Support, Non-U.S. Gov't
Review

MeSH terms

Combinatorial Chemistry Techniques / instrumentation
Combinatorial Chemistry Techniques / methods*
Oligosaccharides / analysis
Oligosaccharides / chemical synthesis*
Oligosaccharides / isolation & purification
Online Systems
Polyethylene Glycols
Resins, Synthetic / chemistry
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization

Substances

Oligosaccharides
Resins, Synthetic
Polyethylene Glycols