Characterisation and molecular cloning of an erythromycin resistance plasmid of Lactococcus lactis isolated from chicken cecum

J Biochem Mol Biol Biophys. 2002 Feb;6(1):7-11. doi: 10.1080/10258140290010151.

Abstract

An erythromycin resistance plasmid, pAJ01 was isolated from Loctococcus lactis isolate C5 that was isolated from a healthy two-week-old chicken cecum. A 4 kb plasmid was transformed into plasmidless L. lactis MG1363 before a restriction endonuclease map was constructed. It was then fused with pUC19 to form pAJ02, which can replicate in Escherichia coli XLI-Blue as well as L. lactis MG1363. The plasmid was stably maintained in Lactococcus for more than 100 generations.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Cecum / microbiology*
  • Chickens
  • Cloning, Molecular
  • Drug Resistance, Bacterial
  • Erythromycin / pharmacology*
  • Escherichia coli / genetics
  • Genetic Vectors*
  • Lactococcus lactis / genetics*
  • Lactococcus lactis / isolation & purification
  • Plasmids / genetics*
  • Restriction Mapping
  • Transformation, Bacterial

Substances

  • Erythromycin