Expression analysis in plant and cell suspensions of CrCKR1, a cDNA encoding a histidine kinase receptor homologue in Catharanthus roseus (L.) G. Don

J Exp Bot. 2002 Sep;53(376):1989-90. doi: 10.1093/jxb/erf048.

Abstract

A full length cDNA (CrCKR1) encoding a hybrid histidine kinase was isolated from a Catharanthus roseus cDNA library. The kinase belongs to the subfamily of cytokinin receptors represented by CRE1/AHK4/WOL in Arabidopsis thaliana. In cell suspensions, the expression of CrCKR1 is not affected by various stress and hormonal treatments but is stimulated in cells continuously exposed to cytokinin. In plants, CrCKR1 is strongly expressed only in the petals of mature flowers. These data suggest that CrCKR1 could take part in the mechanisms leading to the production of secondary metabolites in C. roseus.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Arabidopsis Proteins / genetics
  • Arabidopsis Proteins / metabolism
  • Blotting, Northern
  • Catharanthus / enzymology
  • Catharanthus / genetics*
  • Cells, Cultured
  • Cytokinins / pharmacology
  • DNA, Complementary / chemistry
  • DNA, Complementary / genetics
  • Gene Expression Regulation, Enzymologic / drug effects
  • Gene Expression Regulation, Plant / drug effects
  • Histidine Kinase
  • Molecular Sequence Data
  • Phylogeny
  • Plant Stems / enzymology
  • Plant Stems / genetics
  • Protein Kinases / genetics*
  • Protein Kinases / metabolism
  • RNA, Messenger / drug effects
  • RNA, Messenger / genetics
  • RNA, Messenger / metabolism
  • Receptors, Cell Surface / genetics
  • Receptors, Cell Surface / metabolism
  • Sequence Analysis, DNA

Substances

  • Arabidopsis Proteins
  • Cytokinins
  • DNA, Complementary
  • RNA, Messenger
  • Receptors, Cell Surface
  • Protein Kinases
  • Histidine Kinase
  • WOL protein, Arabidopsis

Associated data

  • GENBANK/AY092025