The purpose of this study was to investigate the presence of a practice effect on the Wingate anaerobic test (WAnT). Twenty-five young adult men (mean age = 20 years) performed 2 trials of the WAnT, which were separated by 7 days. Mean peak power (PP) and mean power (MP) for trials I and II were compared using a 1-way repeated measures analysis of variance to determine if a practice effect existed. Mean PP and MP scores were significantly higher (p < 0.025) on trial II (867.64 and 634.68 W for PP and MP, respectively) than on trial I (764.48 and 604.92 W), indicating that a practice effect occurred. Effect size (Cohen's d) for PP and MP was 0.72 and 0.35, indicating a large effect and small effect, respectively. Therefore, at least 1 full administration should be performed prior to a baseline power output measurement.