Abstract
We report that Brucella abortus and Brucella melitensis agglutinate human (A+ and B+), hamster and rabbit erythrocytes, a heretofore undescribed feature in this genus. This activity was associated with a 29-kDa surface protein (SP29) that bound selectively to these erythrocytes and this binding was inhibited by rabbit anti-SP29 antibodies. Hemagglutination was inhibited by pretreatment of erythrocytes with neuraminidase and by preincubation of B. abortus with chondroitin sulfate, N-acetylneuraminic acid and N-acetylneuramin-lactose.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Amino Acid Sequence
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Animals
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Antigen-Antibody Reactions
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Bacterial Adhesion*
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Brucella / immunology*
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Brucella abortus / immunology
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Chondroitin Sulfates / pharmacology
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Cricetinae
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Erythrocytes / drug effects
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Erythrocytes / immunology*
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Erythrocytes / metabolism
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Hemagglutination Tests
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Humans
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In Vitro Techniques
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Molecular Sequence Data
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N-Acetylneuraminic Acid / metabolism*
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Neuraminidase / pharmacology
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Rabbits
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Receptors, Cell Surface / metabolism
Substances
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Receptors, Cell Surface
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sialic acid receptor
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Chondroitin Sulfates
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Neuraminidase
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N-Acetylneuraminic Acid