Abstract
[2H4]Folic acid was synthesized by deuterating p-aminobenzoic acid, which was then coupled to glutamic acid and 6-formylpterin. Using [2H4]folic acid as starting component enabled the preparation of labeled vitamers tetrahydrofolate, 5-formyltetrahydrofolate, 5-methyltetrahydrofolate, and 10-formylfolate which were characterized by electrospray mass spectrometry and collision-induced dissociation. The mass spectrometric studies confirmed that the compounds could be used as internal standards in stable isotope dilution assays.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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4-Aminobenzoic Acid / chemistry
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Chromatography, High Pressure Liquid
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Deuterium*
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Folic Acid / analogs & derivatives*
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Folic Acid / chemical synthesis*
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Glutamic Acid / chemistry
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Isotope Labeling / methods*
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Leucovorin / chemical synthesis
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Magnetic Resonance Spectroscopy
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Pteridines / chemistry
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Pterins*
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Spectrometry, Mass, Electrospray Ionization
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Tetrahydrofolates / chemical synthesis
Substances
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Pteridines
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Pterins
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Tetrahydrofolates
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10-formylfolic acid
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Glutamic Acid
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5,6,7,8-tetrahydrofolic acid
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2-amino-4-hydroxy-6-formylpteridine
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Folic Acid
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Deuterium
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Leucovorin
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4-Aminobenzoic Acid
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5-methyltetrahydrofolate