Isolation of an antifungal thaumatin-like protein from kiwi fruits

Phytochemistry. 2002 Sep;61(1):1-6. doi: 10.1016/s0031-9422(02)00144-9.

Abstract

A single-chain 21 kDa protein exhibiting antifungal activity against Botrytis cinerea and some suppressive effects on Mycosphaerella arachidicola and Coprinus comatus was isolated from kiwi fruits. The protein, designated kiwi fruit thaumatin-like protein, did not inhibit translation in the cell-free rabbit reticulocyte lysate system but inhibited HIV-1 reverse transcriptase. It was purified to apparent homogeneity using a procedure involving saline extraction, (NH(4))(2)SO(4) precipitation, ion exchange chromatography on DEAE-cellulose, affinity chromatography on Affi-gel blue gel, ion exchange chromatography on SP-Sepharose and gel filtration on Superdex 75, respectively.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Actinidia / chemistry*
  • Amino Acid Sequence
  • Antifungal Agents / chemistry
  • Antifungal Agents / isolation & purification*
  • Antifungal Agents / pharmacology
  • Ascomycota / drug effects
  • Botrytis / drug effects
  • Chromatography, Gel
  • Fruit / chemistry*
  • Inhibitory Concentration 50
  • Plant Proteins / chemistry
  • Plant Proteins / isolation & purification*
  • Plant Proteins / pharmacology*
  • Sequence Homology, Amino Acid
  • Sweetening Agents / chemistry
  • Sweetening Agents / isolation & purification*
  • Sweetening Agents / pharmacology*

Substances

  • Antifungal Agents
  • Plant Proteins
  • Sweetening Agents
  • thaumatin protein, plant