Genotyping on a complementary metal oxide semiconductor silicon polymerase chain reaction chip with integrated DNA microarray

Anal Chem. 2002 Jul 1;74(13):3168-73. doi: 10.1021/ac020053u.

Abstract

A novel method for the fast identification of genetic material utilizing a micro-DNA amplification and analysis device (micro-DAAD) consisting of multiple PCR microreactors with integrated DNA microarrays was developed. The device was fabricated in Si-technology and used for the genotyping of Chinese medicinal plants on the basis of differences in the noncoding region of the 5S-rRNA gene. Successful amplification of the genetic material and the consecutive analysis of the fluorescent-labeled amplicons in the micro-DAAD by the integrated oligonucleotide probes were demonstrated. Parallel analysis was performed by loading the four PCR reactors of the micro-DAAD with different samples of 3-microL volume. Temperature sensors and heating elements of the micro-DAAD enable precise temperature control and fast cycling, allowing the rapid completion of a combined amplification and analysis (hybridization) experiment.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Genotype
  • Microcomputers
  • Microscopy, Confocal
  • Oligonucleotide Array Sequence Analysis / instrumentation*
  • Oligonucleotide Probes
  • Reverse Transcriptase Polymerase Chain Reaction / instrumentation*
  • Spectrometry, Fluorescence

Substances

  • Oligonucleotide Probes