Single-tube genotyping of MBL-2 polymorphisms using melting temperature analysis

U Hladnik; L Braida; M Boniotto; D Pirulli; F Gerin; A Amoroso; S Crovella

doi:10.1007/s102380200014

Single-tube genotyping of MBL-2 polymorphisms using melting temperature analysis

Clin Exp Med. 2002 Jul;2(2):105-8. doi: 10.1007/s102380200014.

Authors

U Hladnik¹, L Braida, M Boniotto, D Pirulli, F Gerin, A Amoroso, S Crovella

Affiliation

¹ Dipartimento di Scienze della Riproduzione e dello Sviluppo, Università di Trieste, Italy.

PMID: 12141527
DOI: 10.1007/s102380200014

Abstract

Recently several authors correlated MBL-2 gene polymorphisms with different pathologies and there is a growing interest for MBL-2 genotyping in a large number of individuals. We have developed a single-tube, rapid, economic, and fully automated melting temperature analysis screening method, based on ABI 7700 Sequence Detection System technology and SYBR Green I chemistry, for the detection of three polymorphisms (exon 1, codons 52, 54, 57) in the MBL-2 gene. We also developed an electronic sheet for the automatic calling of different genotypes, based on the analysis of the first derivative of ABI 7700 raw data.

Publication types

Evaluation Study
Research Support, Non-U.S. Gov't

MeSH terms

Carrier Proteins / genetics*
Genetic Techniques*
Genotype
Humans
Mannose-Binding Lectins
Polymorphism, Genetic*
Temperature

Substances

Carrier Proteins
Mannose-Binding Lectins