Abstract
Cloned cDNA derived from the genome of the virulent type 2 bovine viral diarrhea virus (BVDV) strain NY'93/C was sequenced and served for establishment of the infectious cDNA clone pKANE40A. Virus recovered from pKANE40A exhibited growth characteristics similar to those of wild-type BVDV NY'93/C and proved to be clinically indistinguishable from the wild-type virus in animal experiments. A virus mutant in which the RNase residing in the viral glycoprotein E(rns) was inactivated, revealed an attenuated phenotype. The plasmid pKANE40A represents the first infectious cDNA clone established for a type 2 BVDV and offers a variety of new approaches to analyze the mechanisms of BVDV-induced disease in cattle.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Antibodies, Viral / blood
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Base Sequence
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Bovine Virus Diarrhea-Mucosal Disease / etiology
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Bovine Virus Diarrhea-Mucosal Disease / immunology
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Bovine Virus Diarrhea-Mucosal Disease / virology
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Cattle
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Cloning, Molecular
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DNA, Complementary / genetics
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DNA, Viral / genetics*
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Diarrhea Viruses, Bovine Viral / enzymology
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Diarrhea Viruses, Bovine Viral / genetics*
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Diarrhea Viruses, Bovine Viral / immunology
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Diarrhea Viruses, Bovine Viral / pathogenicity*
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Female
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Fever / etiology
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Leukocyte Count
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Mutation
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Neutralization Tests
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Ribonucleases / genetics
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Ribonucleases / metabolism
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Virulence
Substances
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Antibodies, Viral
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DNA, Complementary
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DNA, Viral
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Ribonucleases