The hydroponics experiment was conducted to study the nutrition basis for growth promotion of wheat by Enhanced Ammonium Nutrition(EAN). Compared with fertilizing NO3- alone, EAN significantly increased N concentration and accumulation in leaves, sheaths, and whole plant, but there were not significant effects on roots. EAN had no significant effects on concentration of P and K, but decreased that of Ca and Mg in whole plant. EAN increased accumulation of P, K, and Ca in whole plant, and decreased that of Mg, whereas single NH4+ decreased accumulation of all mineral nutrients. EAN had different effects on accumulation of mineral nutrients in different genotypes. Compared with fertilizing NO3- alone, EAN increased accumulation of P, K, and Ca of YM158 and LZ953. For the accumulation of JDM, there was little responsive to EAN. These results indicated that EAN promoted N absorption and increased accumulation of N, P, K, and Ca in whole plant.