Bone and bone marrow interactions: hematological activity of osteoblastic growth peptide (OGP)-derived carboxy-terminal pentapeptide. II. Action on human hematopoietic stem cells

Leuk Res. 2002 Sep;26(9):839-48. doi: 10.1016/s0145-2126(02)00008-5.

Abstract

Osteogenic growth peptide (OGP) is a peptide exerting regulatory effects on the bone and on bone marrow. The carboxy-terminal pentapeptide (OGP10-14) is the biologically active portion of OGP. We evaluated OGP10-14 hematopoietic activity performing colony-forming tests on human stem cells derived by bone marrow, peripheral blood and cord blood. Granulocyte-macrophage colony-forming unit (CFU) were significantly increased in OGP10-14-treated samples, while granulocyte-erythrocyte-monocyte-megakaryocyte CFU and burst-forming unit (BFU) erythroid were increased only in the cord blood test.Moreover, OGP10-14 preserves stem cells self renewal potential in long-term culture (LTC) initiating cells and acts directly on CD34+ enriched cells or by increasing activity of stem cell factor (SCF) and granulocyte-megakaryocyte colony-stimulating factor.

Publication types

  • Comparative Study

MeSH terms

  • Antigens, CD34 / analysis
  • Blood Cells / cytology
  • Blood Cells / drug effects
  • Bone Marrow / physiology*
  • Bone Marrow Cells / cytology
  • Bone Marrow Cells / drug effects
  • Bone and Bones / physiology*
  • Cell Differentiation / drug effects
  • Cell Lineage
  • Chemokine CCL4
  • Coculture Techniques
  • Colony-Forming Units Assay
  • Endorphins / pharmacology*
  • Fetal Blood / cytology
  • Granulocyte-Macrophage Colony-Stimulating Factor / pharmacology
  • Hematopoietic Cell Growth Factors / physiology
  • Hematopoietic Stem Cells / cytology
  • Hematopoietic Stem Cells / drug effects*
  • Humans
  • Leukemia / pathology
  • Macrophage Inflammatory Proteins / analysis
  • Myeloid Cells / cytology
  • Organ Specificity
  • Proto-Oncogene Proteins c-kit / analysis
  • Receptors, Granulocyte-Macrophage Colony-Stimulating Factor / analysis
  • Receptors, Interleukin-3 / analysis
  • Stem Cell Factor / pharmacology
  • Stromal Cells / physiology
  • Tumor Cells, Cultured / cytology

Substances

  • Antigens, CD34
  • Chemokine CCL4
  • Endorphins
  • Hematopoietic Cell Growth Factors
  • Macrophage Inflammatory Proteins
  • Receptors, Granulocyte-Macrophage Colony-Stimulating Factor
  • Receptors, Interleukin-3
  • Stem Cell Factor
  • historphin
  • Granulocyte-Macrophage Colony-Stimulating Factor
  • Proto-Oncogene Proteins c-kit