Objective: To determine the common deletion region of loss of heterozygosity (LOH) on chromosome 8p21-8p22 in adenocarcinomas of lung, and facilitate identification of candidate tumor suppressor genes associated with adenocarcinoma of lung.
Methods: PCR and microsatellite analysis were used to examine the LOH frequency of 17 microsatellite loci at the 8p21-8p22 in the samples resected from 32 patients with lung adenocarcinoma and adenosquamous carcinoma. The relationship of LOH for each marker to pathological grade and that to clinical stage are investigated.
Results: Thirty-one out of the 32 (96.67%) samples showed allelic loss in at least one of the 17 markers. The most frequent LOH loci were mainly located in the three regions: D8S254-261, D8S1827-1731, and D8S1135 loci. Among them, the LOH frequency of D8S261 locus was related to the stage of tumor (P < 0.05).
Conclusion: An interval of common deletion on chromosome 8p22, encompassing D8S254-261, D8S1827-1731 and D8S1135, might harbor candidate tumor suppressor gene(s) associated with pathogenesis of adenocarcinoma of lung.