Determination of the subcellular distribution of mercaptoundecahydro-closo-dodecaborate (BSH) in human glioblastoma multiforme by electron microscopy

Abstract

The subcellular distribution of mercaptoundecahydro-closo-dodecaborate (BSH) in glioblastoma multiforme tissue sections of several patients having received BSH prior to surgery was investigated by transmission electron microscopy (TEM) using antibodies against BSH and electron energy loss spectroscopy (EELS) and electron spectroscopic imaging (ESI). These microscopic techniques show that BSH is associated with extracellular structures, the cell membrane as well as with the chromatin in the nucleus.