Resazurin microtiter assay plate: simple and inexpensive method for detection of drug resistance in Mycobacterium tuberculosis

Antimicrob Agents Chemother. 2002 Aug;46(8):2720-2. doi: 10.1128/AAC.46.8.2720-2722.2002.

Abstract

A method for detecting multidrug-resistant Mycobacterium tuberculosis by using a reduction of resazurin is described. Eighty clinical isolates were evaluated against isoniazid and rifampin; results at 7 days were compared with those of the proportion method. Specificity and sensitivity were excellent. The method is simple, inexpensive, and rapid and might be used with other antituberculosis drugs.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Antitubercular Agents / pharmacology*
  • Colorimetry
  • Drug Resistance, Microbial
  • Humans
  • Indicators and Reagents
  • Isoniazid / pharmacology
  • Microbial Sensitivity Tests / methods*
  • Mycobacterium tuberculosis / drug effects*
  • Oxazines*
  • Peru
  • Rifampin / pharmacology
  • Tuberculosis / microbiology
  • Xanthenes*

Substances

  • Antitubercular Agents
  • Indicators and Reagents
  • Oxazines
  • Xanthenes
  • resazurin
  • Isoniazid
  • Rifampin