Characterization of cloned mouse Na+/taurocholate cotransporting polypeptide by transient expression in COS-7 cells

Biosci Biotechnol Biochem. 2002 May;66(5):1116-8. doi: 10.1271/bbb.66.1116.

Abstract

The mouse Na+/taurocholate cotransporting polypeptide transiently expressed in COS-7 cells caused sodium-dependent uptake of [3H]taurocholic acid with Km and Vmax values of 18 microM and 102 pmol/mg protein/min, respectively. This Km value is comparable to that for rat NTCP and higher than that for human NTCP. Substrate specificity was evaluated by measuring inhibitory effects of unlabeled bile acids on [3H]taurocholic acid transport.

Publication types

  • Comparative Study

MeSH terms

  • Animals
  • Base Sequence
  • COS Cells
  • Carrier Proteins / genetics
  • Carrier Proteins / metabolism*
  • DNA Primers
  • Humans
  • Mice
  • Organic Anion Transporters, Sodium-Dependent*
  • Rats
  • Symporters*

Substances

  • Carrier Proteins
  • DNA Primers
  • Organic Anion Transporters, Sodium-Dependent
  • Symporters
  • sodium-bile acid cotransporter