Abstract
Dendritic cells (DC) have been proposed to play a pivotal role in transient immune suppression induced by measles virus (MV) infection. In the present study, we show that DC-induced suppression of T-cell proliferation was not mediated by IL-10 or IFNalpha/beta, which are released following infection of DC, but required cell contacts between MV-infected DC and T cells. Human sera containing neutralizing anti-MV antibodies, as well as anti-MV hemagglutinin (HA) or fusion protein (F) mAbs, were found (i) to reverse suppression and (ii) to restore DC allostimulatory capacity. Interestingly, DC-induced T-cell suppression was associated with both phenotypic and functional DC maturation, as demonstrated by IL-12 production and chemotaxis to MIP-3beta. These data suggest that MV infection turns on the maturation program of DC allowing migration to draining lymph nodes, where potent T-cell immune suppression might be achieved via cell surface expression of HA and F glycoproteins, independently of T cell trans-infection.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Antibodies, Viral / immunology
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Antibodies, Viral / pharmacology
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Antigen Presentation / drug effects
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Antigen Presentation / immunology
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CD4-Positive T-Lymphocytes / immunology*
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Cell Communication
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Cell Differentiation
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Chemotaxis, Leukocyte
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Dendritic Cells / immunology
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Dendritic Cells / virology*
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Giant Cells / virology
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Granulocyte-Macrophage Colony-Stimulating Factor / pharmacology
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Hemagglutinins, Viral / biosynthesis
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Hemagglutinins, Viral / immunology*
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Humans
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Immune Tolerance / immunology
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Immunophenotyping
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Interferon-alpha / antagonists & inhibitors
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Interferon-alpha / immunology
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Interleukin-10 / biosynthesis
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Interleukin-12 / biosynthesis
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Interleukin-12 Subunit p40
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Lymph Nodes / immunology
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Lymphocyte Activation*
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Lymphocyte Culture Test, Mixed
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Measles virus / immunology*
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Measles virus / physiology
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Measles virus / radiation effects
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Protein Subunits
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T-Lymphocyte Subsets / immunology*
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Ultraviolet Rays
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Viral Fusion Proteins / biosynthesis
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Viral Fusion Proteins / immunology*
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Virus Replication
Substances
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Antibodies, Viral
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Hemagglutinins, Viral
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Interferon-alpha
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Interleukin-12 Subunit p40
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Protein Subunits
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Viral Fusion Proteins
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hemagglutinin protein G, measles virus
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Interleukin-10
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Interleukin-12
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Granulocyte-Macrophage Colony-Stimulating Factor