Abstract
Two antifungal peptides with novel N-terminal sequences, designated cicerin and arietin were isolated from seeds of the chickpea (Cicer arietinum), respectively. Both peptides were adsorbed on Affi-gel blue gel and CM-Sepharose and exhibited a molecular weight of approximately 8.2 and 5.6 kDa, respectively. Arietin was more strongly adsorbed on CM-Sepharose than cicerin and manifested a higher translation-inhibiting activity in a rabbit reticulocyte lysate system and a higher antifungal potency toward Mycosphaerella arachidicola, Fusarium oxysporum and Botrytis cinerea. Both were devoid of mitogenic and anti-HIV-1 reverse transcriptase activities.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Amino Acid Sequence
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Antifungal Agents / chemistry
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Antifungal Agents / isolation & purification*
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Antifungal Agents / pharmacology*
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Chromatography, Affinity
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Cicer / chemistry*
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Fungi / cytology
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Fungi / drug effects
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Inhibitory Concentration 50
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Intercellular Signaling Peptides and Proteins
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Molecular Sequence Data
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Molecular Weight
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Peptides / chemistry
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Peptides / isolation & purification*
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Peptides / pharmacology*
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Plant Proteins / chemistry
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Plant Proteins / isolation & purification*
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Plant Proteins / pharmacology*
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Seeds / chemistry
Substances
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Antifungal Agents
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Intercellular Signaling Peptides and Proteins
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Peptides
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Plant Proteins
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cicerin protein, Cicer arietinum
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arietin