Cicerin and arietin, novel chickpea peptides with different antifungal potencies

Peptides. 2002 May;23(5):817-22. doi: 10.1016/s0196-9781(02)00005-0.

Abstract

Two antifungal peptides with novel N-terminal sequences, designated cicerin and arietin were isolated from seeds of the chickpea (Cicer arietinum), respectively. Both peptides were adsorbed on Affi-gel blue gel and CM-Sepharose and exhibited a molecular weight of approximately 8.2 and 5.6 kDa, respectively. Arietin was more strongly adsorbed on CM-Sepharose than cicerin and manifested a higher translation-inhibiting activity in a rabbit reticulocyte lysate system and a higher antifungal potency toward Mycosphaerella arachidicola, Fusarium oxysporum and Botrytis cinerea. Both were devoid of mitogenic and anti-HIV-1 reverse transcriptase activities.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Antifungal Agents / chemistry
  • Antifungal Agents / isolation & purification*
  • Antifungal Agents / pharmacology*
  • Chromatography, Affinity
  • Cicer / chemistry*
  • Fungi / cytology
  • Fungi / drug effects
  • Inhibitory Concentration 50
  • Intercellular Signaling Peptides and Proteins
  • Molecular Sequence Data
  • Molecular Weight
  • Peptides / chemistry
  • Peptides / isolation & purification*
  • Peptides / pharmacology*
  • Plant Proteins / chemistry
  • Plant Proteins / isolation & purification*
  • Plant Proteins / pharmacology*
  • Seeds / chemistry

Substances

  • Antifungal Agents
  • Intercellular Signaling Peptides and Proteins
  • Peptides
  • Plant Proteins
  • cicerin protein, Cicer arietinum
  • arietin