Detection of porcine circovirus 2 (PCV-2) DNA by nested PCR from formalin-fixed tissues of post-weaning multisystemic wasting syndrome (PMWS) pigs in Thailand

Wijit Kiatipattanasakul-Banlunara; Rachod Tantilertcharoen; Kazuhiko Suzuki; Stella Maris Albarenque; Roongroje Thanawongnuwech; Hiroyuki Nakayama; Kunio Doi

doi:10.1292/jvms.64.449

Detection of porcine circovirus 2 (PCV-2) DNA by nested PCR from formalin-fixed tissues of post-weaning multisystemic wasting syndrome (PMWS) pigs in Thailand

J Vet Med Sci. 2002 May;64(5):449-52. doi: 10.1292/jvms.64.449.

Authors

Wijit Kiatipattanasakul-Banlunara¹, Rachod Tantilertcharoen, Kazuhiko Suzuki, Stella Maris Albarenque, Roongroje Thanawongnuwech, Hiroyuki Nakayama, Kunio Doi

Affiliation

¹ Department of Pathology, Faculty of Veterinary Science, Chulalongkorn University, Bangkok, Thailand.

PMID: 12069080
DOI: 10.1292/jvms.64.449

Abstract

A retrospective study for the detection of porcine circovirus 2 (PCV-2) DNA was conducted by nested PCR method in 16 cases of swine post-weaning multisystemic wasting syndrome (PMWS) in Thailand. Histopathology showed characteristic lesions of PMWS and intracytoplasmic viral inclusion bodies in macrophages infiltrating in lymphoid tissues. PCV-2 DNA was detected from formalin-fixed and/or formalin-fixed paraffin-embedded tissues from all pigs with PMWS. The amplified products were digested with Hae III.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Circoviridae Infections / veterinary
Circoviridae Infections / virology*
Circovirus / genetics
Circovirus / isolation & purification*
DNA, Viral / analysis*
Polymerase Chain Reaction*
Swine / virology*
Swine Diseases / virology*
Wasting Syndrome / veterinary
Wasting Syndrome / virology*

Substances

DNA, Viral