A molecular approach for early monitoring of decay basidiomycetes in wood chips

FEMS Microbiol Lett. 2002 May 21;211(1):117-22. doi: 10.1111/j.1574-6968.2002.tb11212.x.

Abstract

A DNA-based method for the early detection and identification of decay basidiomycetes on wood chips is described. From 26 species of wood-decaying basidiomycetes and 20 species of wood-inhabiting ascomycetes, DNA fragments containing the internal transcribed spacers, 5.8S and partial 28S rDNA were amplified by PCR using the ITS1-F-NL2 rDNA primer pair and analysed through sequencing and restriction digestion. A decay basidiomycete-specific restriction fragment length polymorphism (RFLP) pattern was generated by DraI. Using this PCR-RFLP method the detection of decay fungi is possible 4 days after the inoculation of the wood chips with either a single culture or mixed species.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Basidiomycota / classification
  • Basidiomycota / genetics*
  • Basidiomycota / isolation & purification*
  • Basidiomycota / metabolism
  • DNA Primers
  • DNA, Fungal / genetics*
  • DNA, Ribosomal / genetics
  • Mycological Typing Techniques
  • Polymerase Chain Reaction
  • Polymorphism, Restriction Fragment Length
  • Restriction Mapping
  • Sensitivity and Specificity
  • Sequence Analysis, DNA
  • Trees / microbiology*
  • Wood*

Substances

  • DNA Primers
  • DNA, Fungal
  • DNA, Ribosomal