Synthesis, crystal structure, antitumor activity and DNA-binding study on the Mn(II) complex of 2H-5-hydroxy-1,2,5-oxadiazo[3,4-f]1,10-phenanthroline

J Inorg Biochem. 2002 Jun 7;90(3-4):79-84. doi: 10.1016/s0162-0134(02)00416-6.

Abstract

The complex [Mn(L)(NO(3))(2)(H(2)O)(2)] (1) (L=2H-5-hydroxy-1,2,5-oxadiazo[3,4-f]1,10-phenanthroline) was synthesized and characterized by elemental analysis, IR and UV. The crystal and molecular structure of 1 was determined by single-crystal X-ray diffraction; crystal data: light yellow, monoclinic, space group P2(1)/n, Z=4, a=7.432(2) A, b=9.582(3) A, c=23.445(7) A, beta=90.519(5) degrees. The Mn atom in 1 is hexa-coordinated in a distorted octahedral arrangement by two N atoms of the ligand L and four O atoms of two water molecules and two nitrate anions. Biological tests in vitro showed that 1 has significant antitumor activity against HL-60, KB, Hela and BGC-823 cells. The interaction of 1 with calf thymus DNA was investigated by absorption titration, thermal denaturation and viscosity measurements. The results suggest that 1 binds with DNA by intercalating via the ligand L.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Antineoplastic Agents / chemical synthesis
  • Antineoplastic Agents / chemistry
  • Antineoplastic Agents / metabolism
  • Antineoplastic Agents / pharmacology
  • Cattle
  • Crystallization
  • Crystallography, X-Ray
  • DNA / chemistry
  • DNA / metabolism*
  • Humans
  • In Vitro Techniques
  • Manganese / chemistry
  • Manganese / metabolism*
  • Molecular Structure
  • Organometallic Compounds / chemical synthesis*
  • Organometallic Compounds / chemistry
  • Organometallic Compounds / metabolism*
  • Organometallic Compounds / pharmacology
  • Spectrophotometry
  • Tumor Cells, Cultured
  • Viscosity

Substances

  • Antineoplastic Agents
  • Mn(II)2H-5-hydroxy-1,2,5-oxadiazo(3,4-f)1,10-phenanthroline
  • Organometallic Compounds
  • Manganese
  • DNA