Quantitative determination for cytotoxic Friedo-nor-oleanane derivatives from five morphological types of Maytenus ilicifolia (Celastraceae) by reverse-phase high-performance liquid chromatography

Phytochem Anal. 2002 Mar-Apr;13(2):75-8. doi: 10.1002/pca.626.

Abstract

Five different morphological types of Maytenus ilicifolia of the same age and harvested under the same conditions showed distinct accumulations of some friedo-nor-oleananes. A rapid, sensitive and reliable reverse-phase HPLC method (employing an external standard) was used for the determination of the cytotoxic triterpenoids, 20 alpha-hydroxymaytenin, 22 beta-hydroxymaytenin, maytenin, celastrol and pristimerin in each of the five types. Well resolved peaks with good detection response and linearity in the range 1.0-100 micrograms/mL were obtained.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Antineoplastic Agents, Phytogenic / chemistry
  • Antineoplastic Agents, Phytogenic / isolation & purification
  • Brazil
  • Chromatography, High Pressure Liquid / methods*
  • Maytenus / anatomy & histology
  • Maytenus / chemistry*
  • Maytenus / classification
  • Molecular Structure
  • Oleanolic Acid / analogs & derivatives
  • Plant Bark / chemistry
  • Plant Leaves / chemistry
  • Plant Roots / chemistry
  • Reference Standards
  • Sensitivity and Specificity
  • Triterpenes / chemistry
  • Triterpenes / isolation & purification*

Substances

  • Antineoplastic Agents, Phytogenic
  • Triterpenes
  • oleanane
  • Oleanolic Acid