[Protective effects of d-, l-, and dl-3-n-butylphthalide on neuronal damage induced by hypoxia/hypoglycemia in cultured rat cortical neurons]

Yao Xue Xue Bao. 1998 Jul;33(7):486-92.
[Article in Chinese]

Abstract

The effects of l-3-n-butylphthalide(l-NBP) and d-3-n-butylphthalide(d-NBP) on hypoxia/hypoglycemia-induced cytotoxicity in primary cultured rat cortical neurons were studied. l-NBP and d-NBP(1-100 mumol.L-1) were shown to inhibit hypoxia/hypoglycemia-induced LDH release, decrease the percent of cell death and improve the damaged cellular morphology at 10 mumol.L-1 concentration. In addition, l-NBP, d-NBP and dl-NBP were also found to significantly reduce the liberation of polyribosomes from the neuronal rough endoplasmic reticulum and disaggregation of polyribosomes induced by hypoxia/hypoglycemia. These data suggest that l-NBP, d-NBP and dl-NBP can remarkably protect cultured neurons against hypoxia/hypoglycemia induced damage.

Publication types

  • English Abstract
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Benzofurans / pharmacology*
  • Cell Death / drug effects
  • Cell Hypoxia
  • Cells, Cultured
  • Cerebral Cortex / cytology
  • Cerebral Cortex / metabolism*
  • Female
  • Fetus
  • Hypoglycemia
  • L-Lactate Dehydrogenase / metabolism
  • Neurons / metabolism
  • Neurons / ultrastructure
  • Neuroprotective Agents / pharmacology*
  • Polyribosomes / drug effects
  • Rats
  • Rats, Wistar
  • Stereoisomerism

Substances

  • Benzofurans
  • Neuroprotective Agents
  • 3-n-butylphthalide
  • L-Lactate Dehydrogenase