Abstract
The three genes (pduCDE) encoding the diol dehydratase of Lactobacillus collinoides were sequenced. They exhibited strong identities with the ddrABC and pduCDE genes of Klebsiella oxytoca and Salmonella enterica, respectively. These genes are part of a putative operon with at least four other genes. An eighth open reading frame was identified as homologous to the pocR gene (encoding the operon regulatory protein). Although the enzyme was detected in exponential growth phase, PduCDE activity was increased at the end of exponential phase in presence of 1,2-propanediol.
MeSH terms
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Bacterial Proteins / genetics
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Bacterial Proteins / metabolism*
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Base Sequence
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DNA-Binding Proteins / genetics
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Enzyme Induction / drug effects
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Genes, Bacterial*
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Klebsiella / enzymology
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Klebsiella / genetics
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Klebsiella pneumoniae / enzymology
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Klebsiella pneumoniae / genetics
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Lactobacillus / enzymology*
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Molecular Sequence Data
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Open Reading Frames
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Operon*
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Propanediol Dehydratase / genetics
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Propanediol Dehydratase / metabolism*
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Propylene Glycol / pharmacology
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Reverse Transcriptase Polymerase Chain Reaction
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Salmonella enterica / enzymology
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Salmonella enterica / genetics
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Species Specificity
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Trans-Activators*
Substances
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Bacterial Proteins
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DNA-Binding Proteins
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PocR protein, Salmonella
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Trans-Activators
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Propylene Glycol
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Propanediol Dehydratase