Identification of a putative vacuolar serine protease gene in the rice blast fungus, Magnaporthe grisea

Biosci Biotechnol Biochem. 2002 Mar;66(3):663-6. doi: 10.1271/bbb.66.663.

Abstract

We identified and cloned a gene designated SPM1, encoding a serine protease from the rice blast fungus Magnaporthe grisea. SPM1 is a single-copy gene, encoding a subtilisin-like serine protease with 536 amino acids. Analyses of the deduced amino acid sequence of SPM1 suggested that SPM1 would be localized in a vacuole, an important organelle in pathogenicity.

MeSH terms

  • Amino Acid Sequence
  • Ascomycota / enzymology*
  • Ascomycota / genetics*
  • Ascomycota / pathogenicity
  • Base Sequence
  • Cloning, Molecular
  • DNA Primers
  • Genes, Fungal
  • Molecular Sequence Data
  • Oryza
  • Plant Diseases / microbiology*
  • Serine Endopeptidases / genetics*
  • Subcellular Fractions / enzymology
  • Vacuoles / enzymology*

Substances

  • DNA Primers
  • Serine Endopeptidases

Associated data

  • GENBANK/AB066277
  • GENBANK/AI068959
  • GENBANK/AJ251921